Fig. 3.

Evaluation of myelination in the CC by LFB/CV staining in C57BL/6 mice following 6 weeks of the experiment protocol in a NC; b CPZ; c CPZ + DMF; d CPZ + RUT50 and e CPZ + RUT100 groups. Demyelination was induced by CPZ, whereas DMF and RUT therapy abrogated CPZ-induced demyelination and restored the myelinated appearance of the CC. f Quantitative analysis of the percentage area of myelination in the CC by LFB/CV staining. ** p  < 0.01 vs. NC group, ^# p  < 0.05 and ^## p  < 0.01 vs. CPZ group, and ^• p  < 0.05 vs. CPZ + RUT50 (ANOVA). NC: control group fed on normal diet; CPZ: cuprizone group fed on diet enriched with 0.2% (w/w) cuprizone; DMF: dimethyl fumarate 15 mg/kg; RUT50: rutin 50 mg/kg; RUT100: rutin 100 mg/kg; CC: corpus callosum; LFB/CV: luxol fast blue/cresyl-violet. Scale bar: 100 μ