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. 2024 Apr 10;14:8417. doi: 10.1038/s41598-024-58888-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Establishment of IVDD animal model and RT-qPCR (A) Schematic diagram of the puncture-induced IVDD model. (B) Safranin-O staining of normal and IVDD rats. (C–G) Expression levels of RBM15, YTHDF3, HNRNPA2B1, ALKBH5, YTHDC1 in caudal disc tissues of Normal and IVDD groups were detected by RT-qPCR. ****P < 0.0001.