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. 2024 Apr 10;10:171. doi: 10.1038/s41420-024-01941-4

Fig. 1. Overexpression of miR-3074-5p led to adverse pregnancy outcomes in mice.

Fig. 1

A Upper: representative images of fetuses respectively collected from a wild type (WT) pregnant and a miR-3074-5p-knock-in (3074-KI) pregnant mouse at GD13.5 (vaginal plug was defined as GD0.5); below: the embryo resorption rate, fetal weight and placenta weight in GD13.5 pregnant WT (n = 4) and 3074-KI (n = 3) mice. B Upper: representative images of fetuses respectively collected from a WT mouse and a 3074-KI mouse at GD16.5; below: the embryo resorption rate, fetal weight and placenta weight in GD16.5 pregnant WT (n = 3) and 3074-KI (n = 3) mice. C Uterine miR-3074-5p expression in pregnant WT (n = 3) and 3074-KI (n = 3) mice at GD7.5 detected by in situ hybridization (miR-3074-5p, red; nuclei, blue). D Serum miR-3074-5p level in GD7.5 WT (n = 5) and 3074-KI (n = 5) mice detected by qPCR. Experiments were independently repeated three times. E Serum levels of cytokines in GD7.5 WT (n = 4) and 3074-KI (n = 4) pregnant mice determined by the multiplex cytokine arrays. Replicate wells were set for each sample. F Upper: representative images of uterine tissues collected respectively from pregnant mice at GD13.5; below: number of implanted embryos in GD13.5 pregnant mice; NS: pregnant mice were injected with saline at GD3.5 (n = 4), NC: pregnant mice were injected with negative control sequence at GD3.5 (n = 10), miR-3074-5p agomir: pregnant mice were injected with miR-3074-5p agomir at GD3.5 (n = 14). G Serum levels of cytokines in GD7.5 pregnant mice treated with negative control (NC) (n = 3) or miR-3074-5p agomir (miR-3074 agomir) (n = 3) determined by the multiplex cytokine arrays. Two replicate wells were set for each sample. H Uterine miR-3074-5p expression level of GD7.5 pregnant mice treated with NC (n = 3) or miR-3074-5p agomir (n = 3) detected by qPCR. Experiments were independently repeated three times. Uterine levels of IL-1β (I) and TNF-α (J) in GD7.5 pregnant mice treated with NC (n = 3) or miR-3074-5p agomir (n = 3) detected by ELISA. Three replicate wells were set for each sample. All data are presented as means ± SEM, differences were identified by unpaired t test, *p < 0.05, **p < 0.01,***p < 0.001, ns: p > 0.05.