FIGURE 6.

Synergistic inhibitory effects of thiolutin and quizartinib on cellular signaling, cell proliferation, and apoptosis in FLT3‐ITD Ba/F3 cells. (A) The expression levels of p‐STAT5 in ITD cells, following various treatments, were detected by western blot. α‐Tubulin was used as an internal control. The relative expression levels of p‐STAT5 were calculated by the intensities of p‐STAT5 against STAT5, which for cells without treatment with the drug was set as 1.0. Data were analyzed by one‐way ANOVA and presented as the mean ± SD (n = 3). These treatments also affected cell proliferation abilities using cell counting (B) and cell apoptosis using Annexin V‐FITC apoptosis assay kit (C). (D) MV4‐11 cells and RS4‐11 cells were treated with different doses of thiolutin, and a cell viability assay was performed to determine the drug sensitivity. The number of cells without the treatment was set as 100%. *P < 0.05, **P < 0.01, ***P < 0.001.