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. 2024 Mar 28;15:1340691. doi: 10.3389/fphar.2024.1340691

FIGURE 4.

FIGURE 4

Specific decrease in pvalb GABAergic transcripts in PB, but not Lev treated animals. (A) Animals treated with PB, but not with LEV showed significant dysregulation of GABAergic neuron related genes. (B,C) qPCR analysis confirmed that PB treatment significantly reduced both general GABAergic marker gad1 transcripts (One Way ANOVA, F2,21 = 18.97, p-value <0.0001, VEH vs. PB adj. p-value = 0.0007 with Holm-Sidak corrected) and reduction of pvalb transcripts (One Way ANOVA, F2,21 = 43.34, p-value <0.0001, VEH vs. PB adj. p-value <0.0001, Holm-Sidak corrected). (D,E) Representative photomicrographs of parvalbumin (left column) and DAPI (middle column) staining in the hippocampus in animals treated with vehicle (top row), PB (middle row) and LEV (bottom row). Animals treated with PB showed significantly lower levels of immunoreactivity compared to vehicle treated animals (One Way ANOVA, F2,8 = 11.3, p-value <0.0047, adj. p-value = 0.012, Holm-Sidak corrected). CC = corpus callosum, SP = stratum pyramidale of CA1. (F) Representative image showing the pattern of PV expression in a whole hippocampus section with enlarged views of subfields.