Figure 4.

GATA4 is a positive regulator of cGAS-STING-mediated IFNα induction. (A) U937 cells were transfected with a GATA4 expression vector or control empty vector and then stimulated with 2′3′-cGAMP. GATA4, IFNA1, and IFNB expressions were quantified by RT-qPCR. The 2^-ΔΔCT method was used to calculate the relative gene expression, with normalization to GAPDH as the internal control. Results are representative of three independent experiments (mean ± SEM of triplicate RT-qPCR measurements). (B) U937 cells transfected with a GATA4 expression vector or control empty vector were placed into the wells of an IFNα ELISpot plate. The ELISpot plates were processed after 24 h of cell culture with 2′3′-cGAMP. Results are representative of two independent experiments (mean ± SEM, one-way ANOVA and Tukey’s multiple comparison test. ***p<0.001).