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. 2024 Mar 28;12:1355915. doi: 10.3389/fbioe.2024.1355915

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Copyright © 2024 Grady, Castellanos Franco, Schossau, Ashbaugh, Pelled and Gilad.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EPG split NanoLuc experiments in E. coli BL21 cells. Readings were taken on the IVIS every 10 s with an open filter. Electromagnetic stimulus was applied to the cells for 2 min and shown as a shaded region. (A) Illustration of the EPG split NanoLuc construct. (B) A model of the EPG split NanoLuc construct. E. coli lysate (C) and whole-cell E. coli (D) containing the EPG split NanoLuc (red) showed an increase in luminescence in contrast to EPG-truncated (Blue) and --flipped EPG (Black). Data are shown as mean ± S.E.M. Change in luminescence from before and after stimulus of each well in lysate (E) and whole-cell (F) groups is shown with a line at median. The results shown are duplicate experiments with N = 6 wells in each trial. Statistical significance was calculated using an unpaired t-test with Welch’s correction. A (*) denotes p-value <0.05.