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. Author manuscript; available in PMC: 2024 Apr 11.
Published in final edited form as: Cell Rep. 2023 Nov 11;42(11):113421. doi: 10.1016/j.celrep.2023.113421

Figure 3. Molecular characterization and pseudotemporal trajectories of hair cells in cochlear organoids.

Figure 3.

(A) Volcano plot indicating genes differentially expressed between two clusters of hair cells in organoids: clusters 7 (primarily in day 0 organoids) and 8 (primarily in day 10 organoids).

(B) Aggregate expression of markers specifically expressed in E14, E16, P1, or P7 cochlear hair cells in clusters 7 and 8. Orange indicates high expression, whereas gray indicates low expression.

(C) Aggregate expression of genes expressed in cochlear and non-cochlear Atoh1-dependent cell lineages in clusters 7 and 8.

(D) Monocle pseudotime trajectory delineates transdifferentiation of Lgr5+ supporting cells (cluster 4) to hair cells (clusters 7 and 8). Trajectory labeled by cluster number (left) or pseudotime (right). For an interactive version of the trajectory analysis, see https://umgear.org/lgr5org.

(E) Expression of known marker genes with dynamic expression across pseudotime.