Fig. 2.

CsTOC1 and CsLHY gene expression rhythms in adult chestnut under different seasonal conditions. (A and B) Stem material (second-year branch internodes) and leaves, respectively, collected in June. (C and D) Stem material and winter buds, respectively, collected in December. Samples were collected at 3-h intervals. Each experiment was performed at least twice with similar results; one representative data set is shown. The quantification was as described in the legend of Fig. 1. The open and filled bars above each graph represent the natural day and night lengths, respectively, as provided by the National Institute of Meteorology in Madrid.