Fig. 3.

CsTOC1 and CsLHY gene expression rhythms in chestnut stems from 16- to 24-week-old seedlings grown under different conditions of temperature and photoperiod. (A) Stems from seedlings grown at LD [16 h light/8 h dark (16:8)] and 22°C. (B) Stems from seedlings grown for 1 week at LD and 4°C. (C) Stems from seedlings grown at SD (8:16) and 22°C. (D) Stems from seedlings grown for 1 week at SD (8:16) and 4°C. Samples were collected at 3-h intervals. Each experiment was performed at least twice with similar results; one representative data set is shown. The quantification was as described in the legend of Fig. 1. The open and filled bars above each graph represent the lights being on and off, respectively.