Fig. 5.

Recovery of standard performance of the circadian clock in ecodormant and endodormant chestnut seedlings. (A and B) CsTOC1 and CsLHY gene expression rhythms in chestnut stems (A) and leaves (B) from 16- to 24-week-old seedlings grown under standard conditions (LD, 22°C) and subsequently transferred to 4°C for 7 days and placed back on standard growth conditions for 1 week. (C) CsTOC1 and CsLHY gene expression rhythms in chestnut stems from 11-month-old endodormant plants (see Material and Methods) grown under natural conditions in Madrid and subsequently transferred to controlled-environment growth chambers at standard conditions for 1 week. Samples were collected at 3-h intervals. Each experiment was performed at least twice with similar results; one representative data set is shown. The quantification was as described in the legend of Fig. 1.