FIG. 1.

Survival of vaccinated rabbits following ocular challenge with HSV-1 McKrae. Rabbits were vaccinated either i.m. or ocularly with either gB2/gD2 or HSV-1 KOS as described in Materials and Methods. The mock-vaccinated control (Cont) group was vaccinated i.m. with adjuvant alone. Three weeks after the final vaccination, all rabbits were challenged with 2 × 10⁵ PFU of HSV-1 McKrae in both eyes as described in Materials and Methods. Survival was determined 3 weeks after challenge. The ratio above each bar shows the number of surviving rabbits/the number of challenged rabbits. Comparisons were done as follows: periocular HSV-1 KOS group versus mock control group (P = 0.008 [Fisher exact test]; systemic HSV-1 KOS group versus mock control group (P = 0.002); periocular gB2/gD2 versus mock (P = 0.054); systemic gB2/gD2 versus mock (P = 0.14); combined gB2/gD2 groups versus mock (P = 0.036 [double-sided Fisher exact test]; P = 0.02 [single-sided Fisher exact test]).