FIG. 3.

HSV-specific tear sIgA. Rabbits were vaccinated once, either systemically or periocularly, with gB2/gD2 and ocularly challenged 21 days later as described in Materials and Methods. Tears were collected at the times indicated, and the relative amount of HSV-1 gD2-specific sIgA in individual tear samples was determined by ELISA. The arrows indicate vaccination (day −21) and ocular challenge (day 0) as labeled. The asterisk indicates that both vaccine groups had significantly higher ELISA titers than the mock vaccine group (P < 0.05).