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. 1998 Oct;72(10):7722–7732. doi: 10.1128/jvi.72.10.7722-7732.1998

FIG. 7.

FIG. 7

NLS present in pAP mediate nuclear transport of MCP. COS-7 cells were cotransfected with plasmids encoding the HCMV MCP and either wild-type or NLS mutant pAP proteins and analyzed 3 days later by indirect IF assay using anti-MCP (A to D) or anti-N1 (E to H), all as described in the text. Shown are photomicrographs of representative cells coexpressing MCP with pAP (A and E), pAP/NLS1− (B and F), pAP/NLS2− (C and G), and pAP/NLS1,2− (D and H). Note focal pattern of intranuclear MCP fluorescence when expressed with pAP (A).