FIG. 8.

Precursor proteinase can self-interact in the cytoplasm of transfected cells. COS-7 cells were transfected with plasmids encoding different constructs of the proteinase precursor and analyzed 3 days later by indirect IF using anti-N1 (A) or anti-C1 (B to D), all as described in the text. Shown are photomicrographs of representative cells expressing the NLS⁺ S118A.m escort alone (A), the S118A/NLS1,2⁻ target alone (B), and the S118A/NLS1,2⁻ target together with the S118A.m escort (C) or together with the NLS⁻ S118A.m/NLS1,2⁻ escort (D).