Fig. 4.
Tissue-specific expression of IgM, IgD, and IgT in naïve rainbow trout. (A) Northern blot analysis (12 μg) by using RNA from 1-yr-old rainbow trout (Clear Springs strain). Th, thymus; Pn, pronephros; Mn, mesonephros; Sp, spleen; Lv, liver; Int, intestine; Mu, muscle; H, heart; Ts, testis. OSU-142 (OSU) and Hot Creek (HC) spleen samples. (B) RT-PCR analysis of IgM and IgT in select tissues. Hrt, heart; Ms, mesonephros; E1, day-12 embryo; E2, day-22 embryo; YS, yolk sac fry; Pbl, peripheral blood lymphocytes.