Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Apr 11;21:34. doi: 10.1186/s12987-024-00528-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 4 — C9-ALS BMEC-like cells have an increased glucose metabolism and altered mitochondrial respiration A) Healthy donor hi-PSCs derived BMEC-like cells (CTR BMECs) and C9-ALS donor hi-PSCs derived BMEC-like cells (ALS BMECs) confocal images. BMEC-like cells were fixed in 100% MeOH. Scale bar equals 50µM. Images and results represent confocal z-stacks analysed in 3D volume. Glucose-1 transporter (Glut-1) in red and DAPI in blue for the nuclei staining. Error bars represent the standard deviation of triplicate Transwell™ filters. Statistical significance was determined using Student’s unpaired t-test (****p < 0.0001). At least a total of 5 images were acquired per each condition, with a minimum of 3 technical replicates for a total of 3 biological replicates (N = 3, total images per condition = 45). B) Mitochondrial Real-Time ATP rate test was carried out on healthy donor hi-PSCs derived BMEC-like cells (CTR BMEC-like cells), and 3 C9-ALS donors hi-PSCs derived BMEC-like cells (ALS BMEC-like cells). Data were acquired and analysed using the Agilent Technologies Sea-horse platform and software. Oxygen consumption rate (OCR) measurement per cells is represented as a time curse. The addition of the mitochondrial drugs Olygomycin (A) and Rotenone/Antimycin A (B) are indicated in the graph. C) Mitochondrial Real-Time ATP rate test was carried out on healthy donor hi-PSCs derived BMEC-like cells (CTR BMEC-like cells), and 3 C9-ALS donors hi-PSCs derived BMEC-like cells (ALS BMEC-like cells). Data were acquired and analysed using the Agilent Technologies Sea-horse platform and software. Error bars represent the standard deviation of triplicate wells N = 3. Data is plotted as Min-Max Box and Whisker. Statistical significance was determined using Student’s unpaired t-test (****p < 0.0001)