Fig. 4.

Influence of LPS on serum zinc concentration and MT and Zip14 expression in IL-6 knockout (KO) and WT mice. LPS (5 μg/g of body weight) was administered 16 h before the mice were killed. (A) Serum Zn concentrations. Relative abundance of MT-1 (B) and Zip14 (C) mRNAs, respectively. (D) Western analysis of Zip14 expression in liver cytosol from PBS- and LPS-treated mice. Immunolocalization of Zip14 in liver sections from PBS-treated (E), LPS-treated (F), or turpentine-treated (H) mice. (G) Zip14 antibody was incubated with Zip14 peptide solution 1 h before use for immunohistochemistry. Values are means ± SEM (n = 4 mice). Values with different letters are significantly different (P < 0.05).