Fig. 6.

Zip14 expression in HEK 293T cells transfected with mZIP14. Immunolocalization of ZIP14 in cells transfected with ZIP14 (A) or with the vector alone (B). Intracellular labile zinc measured with Fluozin-3AM in medium containing 80 μM of zinc in Zip14 transfected cells (C) or cells transfected with the vector alone (D). (E) Zinc accumulation, measured using ⁶⁵Zn in medium containing 10 μM zinc. Transfection with Zip14 (##) or vector alone (○). Values are means ± SEM (n = 2). (F) Relative abundance of human MT-1 and –2 mRNAs in transfected cells 16 h after incubation in medium with a final concentration of 4 μM of zinc. Values are means ± SEM (n = 3). *, P < 0.001 different from cells transfected with the vector alone.