ELISA analysis of expression of assembled mAb CO17-1A produced in transgenic tobacco lines. ELISA was conducted by using 96-well plates coated with the recombinant Ag GA733-2E. mAbm CO17-1A, 50 μl (2.0 μg/ml) of purified mAb CO17-1A from the hybridoma supernatant; C1, C2, and C3, 50 μl of leaf extracts of tobacco transgenic lines expressing the HC and LC; WT, 50 μl of leaf extract of a WT tobacco.