Table 2.
Regression equations for corn net photosynthetic rate (A), stomatal conductance (Gs), internal CO2 concentration in the substomatal chamber (Ci), leaf transpiration (E), H2O2, malondialdehyde (MDA) and leaf soluble protein concentration as a function of N rates at each inoculation treatment
| Treatment | A (µmol CO2 m−2 s−1) | Gs (mol H2O m−2 s−1) |
|---|---|---|
| Ctl | Ŷ = 17.931 + 0.099x − 0.0003 × 2 (R2 = 0.94**, P.M. = 165 kg N ha−1) | Ŷ = 0.373 + 0.0008x (R2 = 0.90**) |
| Azo | Ŷ = 21.937 + 0.150x − 0.0005 × 2 (R2 = 0.89**, P.M. = 150 kg N ha−1) | Ŷ = 0.354 + 0.003x − 0.000008 × 2 (R2 = 0.78**, P.M. = 188 kg N ha−1) |
| Bac | Ŷ = 22.454 + 0.063x − 0.0002 × 2 (R2 = 0.95*, P.M. = 158 kg N ha−1) | Ŷ = 0.377 + 0.002x − 0.000006 × 2 (R2 = 0.88*, P.M. = 167 kg N ha−1) |
| Azo + Bac | Ŷ = 22.715 + 0.143x − 0.0005 × 2 (R2 = 0.85**, P.M. = 143 kg N ha−1) | Ŷ = 0.389 + 0.003x − 0.000009 × 2 (R2 = 0.91**, P.M. = 167 kg N ha−1) |
| Treatment | Ci (µmol CO2mol air−1) | E (mmol H2O m−2s−1) |
| Ctl | Ŷ = 212.325–0.161x (R2 = 0.70**) | Ŷ = 2.082 + 0.001x (R2 = 0.59**) |
| Azo | ns | Ŷ = 2.297 + 0.001x (R2 = 0.53**) |
| Bac | ns | Ŷ = 2.668–0.004x + 0.00001 × 2 (R2 = 0.85*) |
| Azo + Bac | ns | ns |
| Treatment | H2O2(µmol g−1F.M.) | MDA (nmol g−1F.M.) |
| Ctl | Ŷ = 5126.517–8.250x + 0.028 × 2 (R2 = 0.78**) | Ŷ = 32.195–0.162x + 0.0005 × 2 (R2 = 0.98**) |
| Azo | Ŷ = 4743.616–6.8305x + 0.027 × 2 (R2 = 0.93**) | Ŷ = 29.616–0.128x + 0.0004 × 2 (R2 = 0.91**) |
| Bac | Ŷ = 4651.653–5.050x + 0.017 × 2 (R2 = 0.73**) | Ŷ = 29.775–0.1368x + 0.0004 × 2 (R2 = 0.97**) |
| Azo + Bac | Ŷ = 4557.840–5.025x + 0.018 × 2 (R2 = 0.81**) | Ŷ = 28.816–0.138x + 0.0004 × 2 (R2 = 0.98**) |
| Treatment | Soluble proteins (mg mL−1) | |
| Ctl | Ŷ = 0.911 + 0.008x − 0.000025 × 2 (R2 = 0.98**, P.M. = 160 kg N ha−1) | |
| Azo | Ŷ = 1.069 + 0.008x − 0.000025 × 2 (R2 = 0.96**, P.M. = 160 kg N ha−1) | |
| Bac | Ŷ = 1.034 + 0.007x − 0.00002 × 2 (R2 = 0.96**, P.M. = 175 kg N ha−1) | |
| Azo + Bac | Ŷ = 1.148 + 0.008x − 0.000025 × 2 (R2 = 0.93**, P.M. = 160 kg N ha−1) |
Ctl Control, Azo Single inoculation with A. brasilense, Bac Single inoculation with B. subtilis and Azo + Bac = co-inoculation with A. brasilense and B. subtilis; ns Not significant, P.M. the calculated point of maximum in equation (plateau)
** and *: significant at p < 0.01 and p < 0.05, respectively