Fig. 3.

Macrophage MR deficiency reduced the expansion of the TIMD4^– macrophage population in the healthy aging heart. A Flow cytometric analysis of macrophages from hearts of young, middle-aged, and old MR^flox and MR^LysMCre mice. Cardiac macrophages were identified as positive for CD45, CD11b, CD64 and stratified by MHC-II and TIMD4. FACS-based quantification of B cardiac resident macrophages and C TIMD4^– macrophages. Mean ± SEM, n = 3–12 per group; *p < 0.05