Fig. 5.

Immunofluorescence micrographs of heart sections from old MR^flox and MR^LysMCre mice showing A macrophages (CD68⁺ cells), fibroblasts (PDGFRα⁺ cells), and TIMD4 immunoreactivity. Arrows indicate CD68⁺ and TIMD4⁺ cells. In the aged heart of MR^flox mice, TIMD4^– macrophages were localized predominantly to areas containing a large amount of fibroblasts. B Immunoreactivity for CCL2 in CD68 and PDGFRα-positive areas. Old hearts from MR^LysMCre mice displayed a weak expression of CCL2 in PDGFRα-positive areas. Nuclei were stained with NucBlue™. Mean ± SEM, n = 4 per group; *p < 0.05