FIG. 9.
(A and B) Translation of BZLF1 from bicistronic and tricistronic mRNA. The reporter plasmid pZLUC contained a luc reporter gene transcribed from PZ. The effector plasmids used in this study are indicated on the x axes. This study was carried out in EBV-negative Akata cells transfected with 5 μg each of effector and reporter plasmids under latent conditions. The results shown represent the averages of values obtained from two independent experiments. (C) Zta protein translated in the cells was analyzed by Western blotting. P3HR1, cells were maintained under latent conditions; P3HR1+TPA, cells were treated with TPA and sodium butyrate for 24 h.