TABLE 1.
The non-synonymous mutations observed in cat-derived SARS-CoV-2 sequences WIN-AH-2021-OTH-Kari-0029-OS-1 and NCFAD-2020-0085 relative to the Wuhan-Hu-1 reference sequence (MN908947.3)
| Sample | Gene | Nucleotide mutation | Amino acid mutation |
|---|---|---|---|
| WIN-AH-2021-OTH-Kari-0029-OS-1 | ORF1ab | G1820A | G519S |
| (29,655 nucleotides) (38% GC content) | ORF1ab | C10038T | T3258I |
| ORF1ab | G10540A | M3425I | |
| ORF1ab | G11230T | M3655I | |
| ORF1ab | G11266T | L3667F | |
| ORF1ab | G11521T | M3752I | |
| ORF1ab | C16575T | T5437I | |
| ORF1ab | C17745T | T5827I | |
| ORF1ab | A18102G | H5946R | |
| S | G21777A | G72E | |
| S | G21867T | R102I | |
| S | C22033A | F157L | |
| S | G22661T | V367F | |
| S | G23401T | Q613H | |
| S | C23604G | P681R | |
| ORF8 | T28144C | L84S | |
| N | G28307A | A12T | |
| N | G28878A | S202N | |
| NCFAD-2020-0085 | ORF1a | C1059T | T265I |
| (29,786 nucleotides) (38% GC content) | ORF1a | G8083A | M2606I |
| ORF1a | C10319T | L3352F | |
| ORF1b | C14407T | P314S | |
| ORF1b | C14408T | P314L | |
| ORF1b | A18424G | N1653D | |
| ORF1b | C21304T | R2613C | |
| S | A23403G | D614G | |
| S | G23593T | Q677H | |
| ORF3a | G25563T | Q57H | |
| ORF3a | G25907T | G172V | |
| M | G26775T | A85S | |
| ORF8 | C27964T | S24L | |
| N | C28472T | P67S | |
| N | C28869T | P199L | |
| N | G29402T | D377Y |