TABLE 1.
Bacteriophage, plaque morphology, and genomic characteristics
| Phage name | Soil sample collection site | Isolation method | Plaque morphology | Plaque Sizea (mm) | Approx. shotgun coverage (fold) | No. of 150-bp single-end reads | Genome length (bp) | Genome end characteristic | G + C content (%) | No. of ORFsb | No. of tRNAs | Clusterc |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Albedo | Hudson, WI, 44.984533 N, 92.7545 W | Enriched | Clear with halod | 0.1–1 | 83 | 862,002 | 41,813 | 3′ single-stranded overhang 5′-TCTCCCGGCA-3′ | 66.6 | 71 | 1 (Gln) | EB |
| Kenzers | Greenville, PA, 41.4124 N, 80.3813 W |
Enriched | Clear | 1 | 3,293 | 960,944 | 41,261 | 3′ single-stranded overhang 5′-TCTCCCGGCA-3′ | 66.8 | 70 | 1 (Gln) | EB |
| Swervy | Aston, PA, 39.8657 N, 75.4279 W | Direct | Turbid | 0.5–1 | 325 | 93,814 | 41,510 | 3′ single-stranded overhang 5′-TCTCCCGGCA-3′ | 66.7 | 71 | 1 (Asn) | EB |
| Cranjis | Upper Chichester, PA, 39.856232 N, 75.443149 W | Direct | Turbid | 3.5–4 | 99 | 40,843 | 53,222 | Circularly permutated | 68.9 | 91 | 0 | EC |
| JaimeB | Aston, PA, 39.875331 N, 75.440021 W |
Direct | Clear | 3–4 | 14,635 | 1.8 million | 17,445 | 3′ single-stranded overhang 5′-CCCGCCCCA-3′ | 68.7 | 25 | 0 | EE |
| Stormbreaker | Aston, PA, 39.5215 N, 75.260806 W | Direct | Clear | 1 | 1,073 | 74,440 | 54,050 | Circularly permutated | 60 | 54 | 0 | EKe |
| Fullmetal | Aston, PA, 39.876667 N, 75.441667 W | Direct | Clear | 1–1.5 | 197 | 410,171 | 54,438 | Circularly permutated | 59.8 | 55 | 0 | EKf |
a
Plaque size is based on the measurements of three plaques.
b
ORFs, open reading frames.
c
Clusters were identified using sequence similarities to other Microbacterium phage (5).
d
Indicates a clear middle of the plaque with a diffuse or cloudy edge.
e
Subcluster EK2.
f
Subcluster EK1.
g
"ND” indicates that the TEM was not performed.