Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| AF488-CD19 | BioLegend | Cat. # 115521 |
| PE/DZ594-CD23 | BioLegend | Cat. # 101634 |
| BV421-CD21/35 | BioLegend | Cat. # 123421 |
| BUV395-CD93 | BD Biosciences | Cat. # 745595 |
| BV785-CD11b | BioLegend | Cat. # 101243 |
| APC Cy7-F4/80 | BioLegend | Cat. # 123118 |
| AF647-Ly6G | BioLegend | Cat. # 127609 |
| BV650-CD11c | BioLegend | Cat. # 117339 |
| PE Cy7-CD3e | BioLegend | Cat. # 100320 |
| PE Cy5-CD122 | BioLegend | Cat. # 123219 |
| BV510-CD8α | BioLegend | Cat. # 100752 |
| PE-CD4 | BioLegend | Cat. # 100512 |
| AF700-CD45 | BioLegend | Cat. # 103128 |
| BV605-IA/IE | BD Horizon | Cat. # 563413 |
| BUV661-CD5 | BD Biosciences | Cat. # 741459 |
| BUV395-CD4 | BD Biosciences | Cat. # 563790 |
| PacBlu-CD25 | BioLegend | Cat. # 102022 |
| PE-CD44 | BioLegend | Cat. # 103008 |
| APC Cy7-CD117 | BioLegend | Cat. # 105826 |
| PE Cy7-γδTCR | BioLegend | Cat. # 118124 |
| APC-TCRβ | BioLegend | Cat. # 109212 |
| FITC-NK1.1 | BioLegend | Cat. # 108717 |
| FITC-TER119 | BioLegend | Cat. # 116206 |
| BV605-B220 | BioLegend | Cat. # 103244 |
| PE Cy7-CD43 | BioLegend | Cat. # 143210 |
| PE-CD24 | BioLegend | Cat. # 138503 |
| APC-BP1 | eBioscence | Cat. # 17-5891-82 |
| BV421-IgM | BioLegend | Cat. # 406517 |
| BUV395-IgD | BD Horizon | Cat. # 564274 |
| BV650-CD150 | BioLegend | Cat. # 115932 |
| AF700-CD48 | BioLegend | Cat. # 103426 |
| PE Cy5-CD34 | BioLegend | Cat. # 119312 |
| FITC-CD16/32 | BioLegend | Cat. # 101305 |
| BV785-SCA1 | BioLegend | Cat. # 108139 |
| BV711-F4/80 | BioLegend | Cat. # 123147 |
| BV480-CD117 | BD Biosciences | Cat. # 566074 |
| BUV496-CD123 | BD Biosciences | Cat. # 750813 |
| BV570- CD90.2 | BioLegend | Cat. # 105329 |
| PE CF594-Siglec-F | BD Biosciences | Cat. # 562757 |
| PE Cy5 -CD3 | BioLegend | Cat. # 100310 |
| PE-CD103 | BioLegend | Cat. # 121406 |
| eFluor450-Ly6C | Thermo Fisher Scientific | Cat. # 48-5932-82 |
| BV605-Ly6G | BioLegend | Cat. # 127639 |
| BUV805-CD45 | BD Biosciences | Cat. # 748370 |
| PE Cy7-CD64 | BioLegend | Cat. # 139314 |
| BUV737-CD19 | BD Biosciences | Cat. # 612781 |
| AF700-CD8b | BioLegend | Cat. # 126618 |
| AF647-IgA | SouthernBiotech | Cat. # 1040–31 |
| BUV563-IA/IE | BD Biosciences | Cat. # 748846 |
| FITC-EpCAM | BioLegend | Cat. # 118207 |
| BV785-CD45 | BioLegend | Cat. # 103149 |
| AF647-CD64 | BioLegend | Cat. # 139322 |
| BV650-Ly6G | BioLegend | Cat. # 127641 |
| PerCP-Cy5.5-Ly6C | BioLegend | Cat. # 128011 |
| BV421-CD11b | BioLegend | Cat. # 101235 |
| PE-Cy7-CD11c | BioLegend | Cat. # 117339 |
| AF700-IA/IE | BioLegend | Cat. # 107621 |
| Biotin-CD11c | BioLegend | Cat. # 117304 |
| CARD9 | Cell Signaling Technology | Cat. # 12283S |
| Bcl10 | Santa Cruz Biotechnology | Cat. # sc-5273 |
| CARD9 | Santa Cruz Biotechnology | Cat. # sc-374569 |
| p-p65 | Cell Signaling Technology | Cat. # 3033S |
| p65 | Cell Signaling Technology | Cat. # 8242S |
| Flag M2 | Sigma Millipore | Cat. # F1894 |
| pTXR | Cell Signaling Technology | Cat. # 2351S |
| PKCd | Cell Signaling Technology | Cat. # 9616 |
| pS104-Card9 | This study | This study |
| p65 | Santa Cruz Biotechnology | Cat. # sc-8008 |
| Anti-rabbit HRP | Daco | Cat. # P0448 |
| Anti-mouse HRP | Daco | Cat. # P0447 |
| AF488 anti-mouse | Invitrogen | Cat. # A-21202 |
| Bacterial and virus strains | ||
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| Sabouraud dextrose agar | Thermo Fisher Scientific | Cat. # BD210950 |
| Remel Dermatophyte test medium | Thermo Fisher Scientific | Cat. # R01365 |
| 1X RBC Lysis Buffer | Thermo Fisher Scientific | Cat. # 00433357 |
| Liberase | Roche | Cat. # 385040 |
| DNAse I | Roche | Cat. # 10104159001 |
| ViaKrome Live/Dead dye | Beckman Coulter | Cat. # C36628 |
| Zombie UV Live/Dead | BioLegend | Cat. # 423108 |
| DMEM | Thermo Fisher Scientific | Cat. # 10569044 |
| RPMI with glutamax | Life Technologies | Cat. # 72400–120 |
| GM-CSF | PeproTech | Cat. # 315–03 |
| M-CSF | PeproTech | Cat. # 315–02 |
| IL-3 | PeproTech | Cat. # 213–13 |
| Lipofectamine 3000 reagent | Thermo Fisher Scientific | Cat. # L3000–015 |
| Cas9 | IDT | Cat. # 1081059 |
| YPD broth | Thermo Fisher Scientific | Cat. # DF0428-07-7 |
| WGP | Invivogen | Cat. # tltl-wgp |
| LPS | Invivogen | Cat. # tlrl-peklps |
| Sotrastaurin | Selleckchem | Cat. # S2791 |
| 1x Halt Protease & Phosphatase Inhibitor Single-Use Cocktail | Thermo Fisher Scientific | Cat. # 78442 |
| Pierce Universal Nuclease | Thermo Fisher Scientific | Cat. # 88701 |
| Dynabeads Protein G beads | Life Technologies | Cat. # 10004D |
| Laemmli buffer | BioRad | Cat. # 1610737 |
| Western Lightning Chemiluminescence Reagent | Perkin Elmer Life Sciences | Cat. # NEL104001EA |
| 4% PFA | Thermo Scientific | Cat. # J19943-K2 |
| 1x Hoechst 33342 | Life Technologies | Cat. # H3570 |
| RIPA buffer | Boston BioProducts | Cat. # BP-115 |
| MagStrep XT beads | Fisher Scientific | Cat. # NC0776437 |
| Sulfo-SMCC | Thermo/Fisher | Cat. # 22322 |
| activated KLH | Thermo/Fisher | Cat. # 77600 |
| activated BSA | Thermo/Fisher | Cat. # 77110 |
| Protein G | Cytiva Life Sciences | Cat. # P-00067 |
| Immobilon-P membranes | Millipore Sigma | Cat. # IPVH08130 |
| Tris-Glycince-SDS-Running buffer (10x) | Boston BioProducts | Cat. # BP-150 |
| Transfer buffer (10x) | Boston BioProducts | Cat. # BP-190 |
| Tris Buffered Saline-Tween (20x, with 1% Tween-20, pH 7.4) | Boston BioProducts | Cat. # IBB-181X |
| 4–20% Bio-Rad Mini-PROTEAN TGX Stain-Free Gels | BioRad | Cat. # 4568095 |
| β-mercaptoethanol | Sigma | Cat. # M3148–25ML |
| EDTA | Invitrogen | Cat. # 15575–038 |
| LS columns | Miltenyi Biotech | Cat. # 130-042-401 |
| Brilliant Stain Buffer | BD Biosciences | Cat. # 566349 |
| Critical commercial assays | ||
| CXCL1 ELISA | Thermo Fisher Scientific | Cat. # EMCXCL1 |
| Monocyte isolation kit | Miltenyi Biotech | Cat. # 130-100-629 |
| P3 Primary Cell 4D-Nucleofactor X kit S | Lonza | Cat. # V4XP-3032 |
| BD Cytometric Bead Array (CBA) Mouse IL-6 Flex Set | BD Biosciences | Cat. # 558301 |
| BD Cytometric Bead Array (CBA) Mouse TNFa Flex Set | BD Biosciences | Cat. # 558299 |
| PKCd Kinase Enzyme System | Promega | Cat. # V3401 |
| Chromium Single Cell 3’ Gene Expression kit | 10x Genomics | Cat. # PN-1000120 |
| Deposited data | ||
| Single-cell RNA-sequencing data | This study | GSE210224; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?&acc=GSE210224 |
| Experimental models: Cell lines | ||
| HEK293T | Lab strain, mycoplasma free | N/A |
| Experimental models: Organisms/strains | ||
| Mouse: Card9−/− | Hara et al.17 | N/A |
| Mouse: Card9R101C | This study | N/A |
| Fungi: Candida albicans strain SC5314 | Jatin Vyas Lab (Massachusetts General Hospital) | N/A |
| Fungi: Trichophyton rubrum | Paul Verweij (Radboud University Medical Centre, Netherlands) | Clinical isolate |
| Oligonucleotides | ||
| sgRNA sequences are provided in Table S7 | IDT | N/A |
| Recombinant DNA | ||
| pMD601-CARD92–152/WT | This study | N/A |
| pMD602-CARD92–152/I107E | This study | N/A |
| pMD604- CARD92–152/S104D | This study | N/A |
| pMD605-CARD92–152/R101C | This study | N/A |
| pMD606-CARD92–152/R101C/S104D | This study | N/A |
| pMD613-Bcl10Fl/C29A/C57A | This study | N/A |
| pCDH-CMV | Addgene | Cat. # 72265 |
| pCDH-CMV-CARD9 | This study | N/A |
| pCDH-CMV-CARD9R101C | This study | N/A |
| pCDH-CMV-CARD9S104N | This study | N/A |
| pCDH-CMV-CARD9S104D | This study | N/A |
| Software and algorithms | ||
| GraphPad Prism 7 | GraphPad Software | https://www.graphpad.com/ |
| FlowJo | FlowJo | https://www.flowjo.com/ |
| Harmony | Perkin Elmer | N/A |
| Cell Ranger v.6.0.0 | 10x Genomics | N/A |
| Seurat v4.0.1 | Hao et al.44 | N/A |
| Other | ||