Table 1.
Antigen-binding kinetics of knob domains produced by solid-phase peptide synthesis (aIL2_1, K8 and K57) and recombinant knob domain fusion constructs.
| Construct | k a, M-1s-1 | k d, s-1 | K D, M |
|---|---|---|---|
| aIL2_1 | 6.0 × 105 | 3.2 × 10-3 | 9.0 × 10-9 |
| aIL2_1-SNV-N | 2.3 × 105 | 2.5 × 10-3 | 1.79 × 10-8 |
| aIL2_1-BECN1 | 1.43 × 105 | 1.85 × 10-3 | 2.3 × 10-8 |
| aIL2_1-HBB | 4.7 × 105 | 3.1 × 10-3 | 1.01 × 10-8 |
| K8 | 6.2 × 104 | 2.3 × 10-4 | 3.8 × 10-9 |
| K8-SNV-N | 9.8 × 104 | 4.7 × 10-4 | 4.8 × 10-9 |
| K8-BECN1 | 3.8 × 104 | 2.8 × 10-4 | 7.5 × 10-9 |
| K8-HBB | 2.6 × 104 | 5.1 × 10-4 | 1.95 × 10-8 |
| K57 | 2.3 × 105 | 5.4 × 10-4 | 2.4 × 10-9 |
| K57-SNV-N | 1.55 × 105 | 4.5 × 10-4 | 2.9 × 10-9 |
| K57-BECN1 | 5.2 × 104 | 5.3 × 10-4 | 1.02 × 10-8 |
| K57-HBB | 9.4 × 104 | 5.3 × 10-4 | 5.6 × 10-9 |