FIG. 5.

(a) HTA of viral sequences (C2 through V5) obtained from patient 3. After referring to the phylogenetic tree, a representative sequence from group B or D was selected as a probe against other variants. Probes derived from group B or D sequences were able to easily distinguish sequences of the same group (B or D) from a panel of sequences obtained from different groups in the same patient or from an unrelated sample (lane U). The experiment was repeated three times, using different representative sequences for probe and panel, with similar results. ∗, probe lane; arrow, position of homoduplex migration. (b) The effect of tetanus immunization on viral quasispecies turnover in patient 3, as displayed by HTA. In order to determine the turnover of groups B and D, an HTA was performed with the probes described above. For plasma samples, RT PCR products amplified from approximately 100 copies of HIV-1 RNA templates were used as the driver. For PBMC samples, PCR products amplified from about 40 copies fo HIV-1 genomic DNA were used as the driver. Arrow, position of homoduplex migration.