Extended Data Fig. 8 |. Experimental testing of Compound 22 (Cmpd 22) analogs.

a, Inhibitory activity of 24 commercially available analogs of Cmpd 22 at 20 μM. Uptake experiments were performed with 2–6 technical repeats and are averaged from 3 independent experiments (n = 3). Data are represented as mean±s.e.m. b, Chemical structure of Cmpd 22 and selected analogs. c, d, Concentration response experiments show that Cmpd 22-J, Cmpd 22-R, and Cmpd 22-D have comparable inhibitory potencies as Cmpd 22 (c), while Cmpd 22-P and Cmpd 22-C appear to not inhibit AE1 (d). Concentration response uptake experiments were performed in triplicates and are averaged from 4 independent experiments (n = 4). Apparent potencies are calculated as apparent IC₅₀ (mean) and error bars denote 95% confidence intervals. All uptake data have been normalized to Cmpd 22.