Table 1 |.

Cryo-EM data collection, refinement and validation statistics

	AE1-apo (EMD-26165) (PDB 7TY4)	AE1-bicarbonate (EMD-26168) (PDB 7TY7)	AE1-DIDS (EMD-41082) (PDB 8T6V)	AE1-H2DIDS (EMD-26167) (PDB 7TY6)	AE1-DEPC (EMD-26171) (PDB 7TYA)	AE1-dipyridamole (EMD-41081) (PDB 8T6U)	AE1-NIF (EMD-26169) (PDB 7TY8)
Data collection and processing
Magnification	64,000	64,000	81,000	64,000	81,000	64,000	64,000
Voltage (kV)	300	300	300	300	300	300	300
Electron exposure (e−/Å2)	51.85	59.99	52.09	51.85	51.18	51.69	59.99
Defocus range (μm)	−0.5 to −1.8	−0.5 to −1.8	−0.5 to −1.8	−0.5 to −1.8	−0.5 to −1.8	−0.5 to −1.8	−0.5 to −1.8
Pixel size (Å)	1.076	1.076	1.083	1.076	1.083	1.076	1.076
Symmetry imposed	C2	C2	C2	C2	C2	C2	C2
Initial particle images (no.)	4,357,888	2,660,401	9,728,456	2,460,255	3,156,841	4,488,247	2,977,492
Final particle images (no.)	238,474	173,471	914,784	267,008	191,625	270,791	79,981
Map resolution (Å)	2.99	3.37	2.95	2.98	3.07	3.13	3.18
FSC threshold	0.143	0.143	0.143	0.143	0.143	0.143	0.143
Map sharpening B-factor (Å2)	−150.0	−150.4	−187.0	−121.2	−128.7	−115.3	−107.4
Local resolution range (Å)	2.5–45.6	3.0–50.5	2.5–10.1	2.5–40.9	2.7–36.2	2.7–28.2	2.7–51.5
Refinement
Model composition
Non-hydrogen atoms	8,665	8,584	8,686	8,728	8,674	8,647	8,578
Protein residues	1,032	1,034	1,032	1,032	1,028	1,032	1,032
Ligand	14	15	16	16	16	16	16
R.m.s. deviations
Bond lengths (Å)	0.014	0.013	0.025	0.013	0.027	0.010	0.013
Bond angles (°)	1.644	1.637	1.731	1.644	1.966	1.370	1.649
Validation
Clashscore	3.32	2.71	2.36	3.03	3.28	5.69	3.10
Poor rotamers (%)	0.49	1	0.33	1.44	0.22	0	1.89
Ramachandran plot
Favored (%)	95.02	94.43	97.07	94.82	96.05	97.07	95.8
Allowed (%)	4.98	5.57	2.93	5.18	3.95	2.73	4.20
Disallowed (%)	0	0	0	0	0	0.2	0