FIG. 8.

Intranuclear distribution of ICP0 and ICP0-N/X in infected and transfected cells. Vero cells were infected with wild-type HSV-1 strain 17 (A) or the α0 mutant vEL0-N/X (B) or were transfected with plasmids encoding HSV-1 ICP0 (C), ICP0-N/X (D), or both proteins (E and F). At 7 h postinfection or 48 h posttransfection, wild-type ICP0 and the NH₂-terminal deletion mutant ICP0-N/X were detected by indirect immunofluorescence using rabbit polyclonal antibody CLU7, which recognizes epitopes between aa 312 and 400 of HSV-1 ICP0 (A to D), the affinity-purified rabbit polyclonal antibody α0-N18, which recognizes only the NH₂-terminal 18 aa acids of HSV-1 ICP0 and thus fails to recognize ICP0-N/X (E), or the anti-ICP0 mouse monoclonal antibody H1083, which recognizes both ICP0 and ICP0-N/X (F). Panels E and F show the same field of cells. The secondary antibodies used in this analysis were coupled to either fluorescein isothiocyanate (A to E) or rhodamine (F).