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. 2023 Nov 30;21(4):648–656. doi: 10.1038/s41592-023-02085-6

Fig. 2. Photostability of StayGold variants and reference green-emitting FPs in live cells.

Fig. 2

Plot of intensity versus normalized total exposure time, with an initial emission rate of 1,000 photons s−1 per molecule. FPs were expressed as fusions to H2B in HeLa cells. Illumination intensity, 8.66 W cm−2. The curves shown are representative of three repetitions (n = 3 independent experiments). The statistical values of t1/2 (time for photobleaching from an initial emission rate of 1,000 photons s−1 per molecule down to 500) are shown in Table 1. SG, StayGold. Curves are colored as follows. QC2-6 FIQ (mSG) and QC2-6(PT) (mSG2), green; SG and its tandem dimers, dark green; EGFP, dark blue; mEGFP, blue; mNeonGreen, red; mClover3, black; mGreenLantern, magenta. Intensity-normalized curves are shown in Supplementary Fig. 6.

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