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. 2023 Nov 30;21(4):648–656. doi: 10.1038/s41592-023-02085-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Plot of intensity versus normalized total exposure time, with an initial emission rate of 1,000 photons s⁻¹ per molecule. FPs were expressed as fusions to H2B in HeLa cells. Illumination intensity, 8.66 W cm⁻². The curves shown are representative of three repetitions (n = 3 independent experiments). The statistical values of t_1/2 (time for photobleaching from an initial emission rate of 1,000 photons s⁻¹ per molecule down to 500) are shown in Table 1. SG, StayGold. Curves are colored as follows. QC2-6 FIQ (mSG) and QC2-6(PT) (mSG2), green; SG and its tandem dimers, dark green; EGFP, dark blue; mEGFP, blue; mNeonGreen, red; mClover3, black; mGreenLantern, magenta. Intensity-normalized curves are shown in Supplementary Fig. 6.

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