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. 2023 Nov 30;21(4):648–656. doi: 10.1038/s41592-023-02085-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, Confocal images of td5StayGold(c4)=GianCreg (green) and DAPI (cyan) in fixed HeLa cells. A MIP image (20 slices, 1.0-μm z step). Low (left) and high (right) magnifications. Scale bars, 20 μm. b, Volumetric and continuous imaging of HeLa cells expressing td5StayGold(c4)=GianCreg by SDSRM (SpinSR10) revealed the occurrence of fast-moving tubular structures that emerged from the Golgi apparatus (yellow arrows). Two independent experiments (left and right). Scale bars, 5 μm. See Supplementary Video 5. Similar results were obtained from 17 other independent cultured cell samples. c, A MIP of HeLa cells expressing COX8a=mStayGold. Cells were 3D scanned with a z step size of 0.11 μm over an axial range of 2.08 μm by lattice SIM (Elyra 7). SIM² was used for image reconstruction. This MIP corresponds to the image at t = 126 s in Supplementary Video 9. Scale bars, 10 μm, 1 μm (inset). Representative of n = 3 independent samples.