Fig. 4.

IL-1 α and CD8 depletion induce similar changes in lung transcriptomic signatures following RSV infection. Lung RNA extracted from mice receiving α CD8 α , α IL-1 α or IgG monoclonal antibody infected with RSV (RSV) as well as naïve (no treatment) underwent bulk RNASeq. Principal component analysis was carried out to identify any batch effect using the package pca and function prcomp in R (A). Volcano plot showing genes differentially expressed in mice infected with RSV compared with naïve (B), α CD8 α monoclonal treated mice compared to RSV infected control (C), α IL-1 α monoclonal antibody treated mice compared to RSV infected control (D) All plots generated using package Enhancedvolcano packagIn R). (E) PGSEA analysis of the groups using Reactome curated dataset in R version 1.30.0. (F) 102 significant differentially expressed genes (DEGs) important for inducing weight loss response were identified as up regulated in RSV compared to naïve and downregulated in both α CD8 α treated and α IL-1 α treated compared to RSV. The significant DEGs were used for Network analysis using NetworkAnalyst (version 3.0) using InnateDB curated interactions. Red colors indicate an increasing number of connections.