Fig. 7.

RSV infection alters increases the levels of cytokines in the brain by increasing permeability. Brian supernatants were tested against a panel of cytokines (A, C), with a focus on the IL-1 α level (B, D). For RSV study, brain collected from naïve mice, or α CD8 α , α IL-1 α and or control Ig treated during RSV infection (A, B). For IL-1 α intranasal study, brain collected from PBS i.n. and IL-1 α i.n. treated mice. RNAseq was performed on brains from RSV infected and control (naïve) mice. Brain supernatant was collected on day 1, 3, 5, and 7 following infection and an IL-1 α ELISA was performed (E). 6-8 weeks old mice were infected with RSV and 200μl 2% Evans blue was injected intravenously on day 7 of infection 1 hour before the brains were extracted (F) and Evans blue absorbance was quantified using florescence absorbance excitation 485, emission 680 (G). N≥4, each dot represents an individual mouse (B, D-E, G); or mean +/-SEM (A, C). Significance calculated by ordinary one-way ANOVA and post test. *P  ≤  0.05, **P  ≤  0.01, ***P  ≤  0.001, ****P  ≤  0.0001. Experiment was repeated twice.