Fig. 4.

Boxplot of fold changes of SASP mRNAs (n = 8–11 per group). A Nfkb gene expression, B Cxcl1 gene expression, C Ptgs2 gene expression, D Mcp1 gene expression, E Stat3 gene expression, and F Il1β gene expression in female wild-type mice treated with DSS and DSS + Fisetin. The 2^−ΔΔCt method was used to calculate and normalize the gene expression, in which − ∆∆Ct was calculated as − ∆∆Ct = average (∆Ct_control) − ∆Ct_sample and ∆Ct = Ct_{target gene} − Ct_{housekeeping gene}. The housekeeping gene was β2M. Nonparametric multiple pairwise Dunn’s test was performed to evaluate the differential gene expression among different treatments with Bonferroni adjustment applied to control for type I error