Fig. 5. Dimeric L–P polymerase of hPIV3.

a Molecular weight determination of hPIV3 L–P complex by analytical size-exclusion chromatography (SEC). mAU, milliabsorbance units. The theoretical molecular weight of the L–P complex (L–P₄) dimer is 1,058 kDa. b Molecular weight determination of hPIV3 L–P complex by size-exclusion chromatography with multi-angle light scattering (SEC-MALS). LS, light scattering; UV, ultraviolet. c 2D classification of the putative entire L–P dimers in single particle reconstruction by cryo-EM. Light blue arrows indicate the potential parts of other domains to bridge two L cores composed of RdRp-PRNTase domains.