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Detection of DNA fragmentation associated with CD8+ apoptotic cells following stimulation for 36 to 48 h in the presence of anti-CD3. Apoptotic CD8+ cells were detected by the TUNEL assay followed by the addition of streptavidin-Tricolor. The percent apoptotic cells was determined from the cell numbers present in the M1 region. The profiles are from individual mice and are representative of at least two mice tested per time point.
