Fig. 1.

Genes involved in antigen presentation and IFNγ pathways provide protection against NK cell cytotoxicity. (A) Cytotoxicity assay with WT K562 at various E:T, combined with blocking antibody against NKp30, showing the percentage protection (bars) and the specific lysis (dots). (B) Overview of the experimental design. Cas9-expressing K562 cell clones were established and transduced with gRNA library and cocultured together with polyclonally activated NK cells. Next-generation sequencing revealed the difference in distribution of gRNAs between surviving cells and unexposed control culture. (C) Depleted and enriched gRNAs from one replicate in the genome-wide CRISPR screen through analysis using MaGeCK software. Depleted genes involved in IFNγ signaling or antigen presentation are highlighted in blue or red, respectively. (D) Protein interaction analysis of significantly depleted gRNAs, displaying clusters of genes involved in IFNγ signaling and antigen presentation. (E) Gene Ontology biological processes results of significantly depleted gRNAs.