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. 2024 Apr 1;121(15):e2316447121. doi: 10.1073/pnas.2316447121

Fig. 3.

Fig. 3.

TMEM30A KO cells display extracellular PtdSer expression and trigger lower NK cell degranulation and cytokine production. (A) Staining for NK cell receptor ligands of WT K562 cells (blue) and TMEM30A KO cell line (green). (B and C) Degranulation assay with WT K562 cells and TMEM30A KO cell line, showing the percentage of CD107+ cells among CD56dim NK cells (B) or among CD56dim NKG2A− NK cells (C) (n = 12). (D–F) Intracellular cytokine staining assays with WT K562 cells and TMEM30A KO cells, showing the percentage of IFNγ+ cells among CD56 dim NK cells (D) or TNFα+ cells among CD56 dim NK cells (E) (n = 12), with representative density plots (F). (G) Annexin-V staining of live TMEM30A KO K562 cells, compared to WT K562 cells. n indicates experiments with NK cells from different donors. Error bars represent SEM, and the paired t-test was used for statistical analyses.