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. Author manuscript; available in PMC: 2024 Apr 12.
Published in final edited form as: Nat Protoc. 2022 Oct 19;18(1):58–80. doi: 10.1038/s41596-022-00753-z

Fig. 1: Live-cell imaging of acutely dissociated hPSCs.

Fig. 1:

a, Nanolive (3D Cell Explorer) microscope enabling label-free cell imaging. b, Fast-acting CEPT is cytoprotective and maintains cell morphology of WA09 dissociated cells, whereas ongoing cell contractions lead to abnormal cells in the presence of Y-27632. Representative image was taken 5 minutes after cell dissociation. c, Monitoring the morphology of dissociated hPSCs and treatment with CEPT. Images were taken every minute for the first 9 minutes after cell dissociation and plating into the well. Scale bars, 10 µm (b) and 5 µm (c).