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. Author manuscript; available in PMC: 2024 Apr 12.
Published in final edited form as: Nat Protoc. 2022 Oct 19;18(1):58–80. doi: 10.1038/s41596-022-00753-z

Fig. 6: Karyotype analysis, whole-exome sequencing, and multi-lineage differentiation of clonal cell lines.

Fig. 6:

a, Three representative examples of clonal cell lines (WA09) that maintained normal karyotypes after 32 passages. Single-cell cloning, clone-picking, cell line establishment, cell expansion, and routine passaging were performed by using the CEPT cocktail. b, WES variant, genotype, and CNV analysis show genetic stability of WA09 and LiPSC-GR1.1 passaged with CEPT for 20 passages. COSMIC variants by passage number in cancer hotspots show no change in rarity or genotype over passaging. c, Genotype frequencies in WA09 cells by passage number for all SNPs are proportionally constant. Figures b and c were adapted with permission from ref. 21. d, EB differentiation potential of non-clonal parental line (WA09) and derived clonal cell lines measured by using Scorecard analysis. e, f, Immunocytochemistry and immunoblot analysis of non-clonal parental (N) and derived clonal cell lines (1, 5, and 8) after directed differentiation into ectoderm, mesoderm, and endoderm. Scale bar, 200 µm.