Table 1.
Antibodies used for clonal cell line characterization
| Antibody | Manufacturer | Cat. No. | Dilution | RRID |
|---|---|---|---|---|
| Anti-OCT4 | Santa Cruz Biotechnology | SC-5279 | ICC:1:200, WB:1:50 | AB_628051 |
| Anti-SOX2 | R&D Systems | AF2018 | ICC:1:100, WB:1:50 | AB_355110 |
| Anti-NANOG | Cell Signaling Technology | 4903S | ICC:1:200, WB:1:50 | AB_10559205 |
| Anti-beta Tubulin | Novus | NB600–936 | WB:1:5000 | AB_10000656 |
| Anti-SOX17 | Cell Signaling Technology | 81778 | ICC:1:1000, WB:1:50 | AB_2650582 |
| Anti-PAX6 | Biolegend | 901301 | ICC:1:200, WB:1:50 | AB_2565003 |
| Anti-Brachyury | Cell Signaling Technology | 81694 | ICC:1:1000, WB:1:50 | AB_2799983 |
| Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 | Thermo Fisher Scientific | A10037 | ICC:1:1000 | AB_2534013 |
| Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | A21206 | ICC:1:1000 | AB_2535792 |
| Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 | Thermo Fisher Scientific | A32849 | ICC:1:1000 | AB_2762840 |