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. Author manuscript; available in PMC: 2024 Apr 12.
Published in final edited form as: Neuropharmacology. 2020 Dec 1;184:108415. doi: 10.1016/j.neuropharm.2020.108415

Fig. 2. 5-HT transiently increases the frequency of GABAergic sIPSCs through the activation of 5-HT3 receptors and ethanol potentiates the increase induced by a selective 5-HT3 agonist.

Fig. 2.

A. The GABAA receptor antagonist gabazine suppressed all sIPSCs as well as the response to 5-HT. Scale bar = 100 pA, 30 s. B, C. The response to 5-HT was blocked in the presence of the selective 5-HT3R antagonist MDL 72222 while baseline sIPSCs remained (n = 5). Scale bar = 200 pA, 30 s (unpaired t-tests *P < 0.05). D. Representative sIPSC waveforms in response to 5-HT in the absence and presence of 80 mM EtOH. sIPSCs induced by 5-HT. Scale bar = 200 pA, 30 s. E, F. No difference was observed in the charge transfer and peak frequency of sIPSC responses to 5-HT before and during EtOH incubation (n = 5). G. Representative sIPSC waveforms in response to the selective 5-HT3 receptor agonist, mCPBG in the absence and presence of 80 mM EtOH. Scale bar = 200 pA, 30 s. H, I. EtOH potentiates the charge transfer and peak frequency of the 5-HT3 agonist-induced increase in sIPSCs (paired t-tests *P < 0.05, n = 10).