FIG. 1.

Schematic representation of the experimental strategy to generate recombinant B19-lacZ vectors. The recombinant plasmid pCMVp-lacZ, containing the lacZ gene under control of the CMV promoter inserted between the AAV ITRs, has been previously described (21, 23). Recombinant helper plasmids pSP-42 and pSP-46 contain the AAV rep genes under control of their authentic promoters, but the AAV cap genes have been replaced by the B19 cap genes (VP2 and VP1+VP2, respectively) under control of the CMV promoter and flanked by the adenovirus ITRs. The rest of the steps in generating the recombinant B19-lacZ vectors are described in Materials and Methods.