FIG. 3.
HIV-1 DNA detection in TH1 and TH2 clones. Lysates were prepared 24 h p.i. A primer pair was used to detect the earliest region of DNA formed by reverse transcription (141 bp of the minus strong-stop strand). Another primer pair was used to detect full-length HIV-1 DNA (200 bp of LTR-gag). The sense primers of each pair were radiolabeled. Three different TH1 (H1.12, H1.15, and H1.18) and TH2 (H2.25, H2.29, and H2.33) clones shown for each infection are representative of three separate experiments. Tenfold serial dilutions of the ACH-2 cell line, containing one integrated copy of HIV-1 DNA per cell, were made in a background of uninfected T cells and shown for estimation of copy number.