TABLE 2.

Phenotypic summary of T_H cell clones derived from two different human donors^a

Clone	Type	Lymphokineb					Chemokine receptorsc
		IFN-γ	IL-4	IL-5	IL-13	CXCR1	CXCR2	CXCR4	CCR1	CCR5
H1.1	TH1	+	−	−	−	−	+	+	+	+
H1.2	TH1	+	−	−	−	−	+	+	+	+
H1.3	TH1	+	−	−	−	−	+	+	−	+
H1.5	TH1	+	−	−	−	±e	+	+	+	+
H1.11	TH1	+	−	−	−	−	−	NDd	ND	ND
H1.12	TH1	+	−	−	−	−	+	+	+	+
H1.15	TH1	+	−	−	−	−	−	+	+	+
H1.18	TH1	+	−	−	−	ND	+	ND	ND	ND
H2.1	TH2	−	+	+	+	−	+	+	+	+
H2.2	TH2	−	+	+	+	−	+	+	−	+
H2.5	TH2	−	+	+	+	−	+	ND	ND	ND
H2.8	TH2	−	+	+	+	−	+	ND	ND	ND
H2.10	TH2	−	+	+	+	−	+	+	+	+
H2.11	TH2	−	+	+	+	−	+	+	+	+
H2.15	TH2	−	+	+	+	ND	ND	ND	ND	ND
H2.25	TH2	−	+	+	+	−	+	+	+	+
H2.29	TH2	−	+	+	+	−	+	+	−	+
500.F7	TH0	+	+	+	+	−	+	+	+	+
500.G3	TH0	+	+	+	+	−	+	+	+	+
500.E10	TH0	+	+	+	+	−	+	ND	ND	ND
100.F10	TH0	+	+	+	+	−	+	+	+	+

^aHuman T-cell clones were obtained through limiting-dilution analysis as described in Materials and Methods. All of the H-series clones are TTx specific, while the remaining clones are KLH specific. 

^bSupernatants obtained from activated T-cell clones were tested for the production of various cytokines by ELISA, and cytokine mRNA expression was tested by RT-PCR analysis as described in Materials and Methods. 

^cBoth quiescent and activated T-cell clones were examined by flow cytometric analysis for the presence of various chemokine receptors on their cell surface. In addition, many of these clones were also examined by radiolabeled binding assays for chemokine binding sites and affinity as described in Materials and Methods. 

^dND, not determined. 

^e±, not consistently positive.