TABLE 3.
Chemokine receptor expression by human TTx-specific TH1 and TH2 clonesa
| Clone | % Positive (mean channel fluorescence)b
|
|||||
|---|---|---|---|---|---|---|
| Control | CXCR1 | CXCR2 | CXCR4 | CCR1 | CCR5 | |
| H1.1 | 4.2 (3.2) | 3.5 (3.4) | 88.9 (42.2) | 98.7 (106.7) | 43.5 (38.6) | 55.3 (96.2) |
| H1.3 | 2.1 (4.3) | 2.7 (4.4) | 87.4 (37.8) | 96.5 (118.7) | 3.3 (5.6) | 43.2 (94.1) |
| H1.15 | 5.6 (3.5) | NDc | 6.7 (4.2) | 97.4 (123.5) | 49.6 (46.2) | 64.7 (98.2) |
| H1.5 | 2.1 (3.2) | 11.4 (3.6) | 67.4 (54.2) | 99.2 (114.2) | 52.3 (37.2) | 49.6 (76.4) |
| H2.5 | 1.4 (2.4) | 2.2 (2.4) | 78.9 (32.1) | 97.6 (122.3) | ND | 69.5 (96.2) |
| H2.25 | 3.2 (1.6) | 3.2 (2.1) | 84.3 (42.3) | 98.9 (118.7) | 39.8 (34.5) | 54.3 (78.6) |
| H2.29 | 2.4 (2.6) | 2.6 (2.2) | 89.5 (45.7) | 99.2 (123.4) | 5.8 (3.6) | 88.4 (97.2) |
a
A total of 106 cells were suspended in PBS and stained with fluorescein isothiocyanate-labeled MAb to CXCR1, CXCR2, CXCR4, CCR1, or CCR5. After staining, the cells were washed and then fixed with 1% paraformaldehyde.
b
All were analyzed on a FACStar Plus flow cytometer.
c
ND, not determined.