TABLE 7.

Levels of HIV gag mRNA/10⁵ viable cells in T_H1 and T_H2 clones after HIV-1 infection

Clonea	Type	Antigen specificity	HIV gag RNA (mol/105 viable cells)b
			Day 7	Day 14
H1.19	TH1	TTx	7,300	108,000
H1.19	TH1	PMA–anti-CD3	8,400	205,000
H1.15	TH1	TTx	12,700	231,000
H1.18	TH1	TTx	9,700	196,000
H2.29	TH2	TTx	4,600	87,000
H2.33	TH2	TTx	5,300	95,000
H2.25	TH2	TTx	3,800	79,000

^aDerivation, antigen activation, and HIV_IIIB infection of T-cell clones were performed as described in Materials and Methods. The H1.19 clone was not previously described. 

^bConstruction and use of an RNA standard and RT-PCR analysis are described in Materials and Methods. For quantitation of number of HIV gag mRNA molecules, the gel was scanned on a PhosphorImager (Molecular Dynamics), using ImageQuant and Microsoft Excel programs, in comparison to a standard curve on each gel. Viable cells were separated by Ficoll-Hypaque density centrifugation.